Fig 8.
Effects of iron on expression of the mntH gene in E. coli and B. japonicum
Cells were grown to mid log phase, then 0 or 100 µM FeCl3 was added to the medium, and cells were harvested after 30 minutes. (A) Analysis of mntH mRNA by qPCR in E. coli wild type (Wt) or oxyR strain. (B) Analysis of mntH mRNA in B. japonicum cells. The data are expressed as the relative starting quantity (SQ) of mntH mRNA normalized to the housekeeping gene gapA, and are presented as the average of triplicate samples with the error bars representing the standard deviation.