Table 3.

Determination of intracellular manganese and iron content of E. coli and B. japonicum wild type and oxyR strains. Data are expressed as average nmol metal per mg protein ±standard deviation.

		E. coli		B. japonicum
Medium (μM)		nmol Mn/mg protein		nmol Mn/mg protein
Mn	Fe	Wt	oxyR	Wt	oxyR
0	0	0.33 ±0.01	0.38 ±0.02	1.57 ±0.03	1.38 ±0.04
0	20	0.62 ±0.01	0.53 ±0.01	1.47 ±0.17	1.53 ±0.08
0	100	0.91 ±0.04	0.70 ±0.01	1.53 ±0.05	1.51 ±0.04
20	0	2.6 ±0.1	3.1 ±0.2	13.2 ±0.2	10.7 ±0.2
20	20	27 ±2	1.7 ±0.1	12.8 ±0.2	11.1 ±0.2
20	100	102 ±1	2.1 ±0.1	11.6 ±0.2	10.2 ±0.2
100	0	67 ±1	64 ±5	16.0 ±0.3	16.7 ±0.8
100	20	220 ±4	73 ±1	17.6 ±0.6	16.8 ±0.3
100	100	311 ±2	56.3 ±0.1	16.9 ±0.4	16.2 ±0.3

Medium		nmol Fe/mg protein		nmol Fe/mg protein
Mn	Fe	Wt	oxyR	Wt	oxyR
0	0	2.11 ±0.03	1.94 ±0.06	1.38 ±0.01	1.42 ±0.04
0	20	222 ±2	204 ±1	47 ±0.2	46 ±2
0	100	1132 ±8	1063 ±8	233 ±3	206 ±3
20	0	2.12 ±0.01	2.02 ±0.01	1.10 ±0.00	1.17 ±0.05
20	20	212 ±2	208 ±2	53 ±1	47 ±1
20	100	1255 ±14	1192 ±38	239 ±16	229 ±16
100	0	2.24 ±0.06	1.85 ±0.12	1.26 ±0.02	1.15 ±0.04
100	20	210 ±2	212 ±2	55 ±3	48 ±1
100	100	1236 ±20	1196 ±12	248 ±2	228 ±3