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. Author manuscript; available in PMC: 2016 Aug 5.
Published in final edited form as: J Am Chem Soc. 2015 Jul 17;137(30):9587–9594. doi: 10.1021/jacs.5b02074

Figure 1.

Figure 1

A single KF nanocircuit and the chemically modified dNTPs tested for their incorporation by KF. (a) A schematic diagram of a single-walled carbon nanotube field effect transistor (SWCNT-FET) non-covalently bio-conjugated to a single molecule of DNA polymerase I (KF) through a single cysteine introduced in the “fingers” subdomain. A pyrene-maleimide linker (yellow) adhered to the SWCNT-FET through π-π stacking and covalently attached to the single cysteine to immobilize the KF. The SWCNT-FET was grown on SiO2, connected to source and drain metal electrodes, and passivated with a polymer (PMMA, red). (b) Atomic force microscopy shows the 1–2 nm diameter of the SWCNT FET with a single KF attachment (7 nm, arrow). (c) Chemical structures of representative dNTP analogs with chemical modifications from the native dNTPs highlighted in red.