. 2011 Jul 21;63(2):127–129. doi: 10.1016/S0377-1237(07)80054-2

Table 1.

Biochemistry of cofactor binding to hLDH and pLDH at pH 9.2 and 25°C

Cofactor	hLDH isozyme A4		hLDH isozyme B4		Recombinant pLDH
K_cat	K_m	K_cat	K_m	K_cat	K_m
(per min)	(μM)	(per min)	(μM)	(per min)	(μM)
NAD⁺	961,000	93	630,000	37	241,000	210
APAD⁺	19,000	56	4,600	37	2,280,000	120

⁺

Nicotinamide adenine dinucleotide (NAD)

⁺

3 acetylpyridine adenine dinucleotide (APAD)