Fig. 9.

Formulation F8 elicits non-inferior immune response compared to bolus injections of IPV. Humoral immune response elicited by a single bolus of IPV (Δ open triangle), a single bolus of IPV co-injected with empty particles (✕ cross), two boluses of IPV (☐ open square), or IPV encapsulated in F8 microspheres (■ solid square) was measured in rats. Antibody titers specific for type 1 (A), type 2 (B), or type 3 (C) poliovirus are plotted on a log-2 scale as geometric mean ± 95% confidence interval. Statistically significant differences from the positive control (two boluses of IPV) are marked as: * < 0.05 and ** < 0.01 (IPV encapsulated in F8 microspheres); § < 0.05 and §§ < 0.01 (single bolus co-injected with empty particles); and # < 0.05 and ## < 0.01 (single bolus).