Table 3.
IPV encapsulation efficiency in microspheres. Percent encapsulation efficiency is normalized to theoretical initial loading. Percent recovery of D-antigen during release is normalized to the actual measured loading. Data are represented as mean ± standard deviation (n = 3).
| Formulation |
IPV encapsulation efficiency (%) |
Total IPV encapsulated (number of doses/50 mg) |
D-antigen recovery during release (%) |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Type 1 | Type 2 | Type 3 | Type 1 | Type 2 | Type 3 | Type 1 | Type 2 | Type 3 | |
| F1 | 84 ± 4 | 81 ± 5 | 77 ± 2 | 27 ± 1 | 26 ± 2 | 25 ± 1 | 2.3 ± 0.3 | 4.6 ± 0.4 | 4.1 ± 0.3 |
| F2 | 69 ± 4 | 67 ± 4 | 67 ± 5 | 22 ± 1 | 21 ± 1 | 21 ± 2 | 2.7 ± 0.1 | 5.6 ± 0.6 | 4.2 ± 0.2 |
| F3 | 68 ± 7 | 69 ± 11 | 64 ± 6 | 22 ± 2 | 22 ± 3 | 20 ± 2 | 4.6 ± 0.2 | 9.1 ± 0.4 | 4.9 ± 0.3 |
| F4 | 66 ± 5 | 63 ± 6 | 70 ± 6 | 21 ± 1 | 20 ± 2 | 22 ± 2 | 2.4 ± 0.3 | 23 ± 1.2 | 2.2 ± 0.2 |
| F5 | 74 ± 4 | 75 ± 4 | 69 ± 5 | 24 ± 1 | 24 ± 1 | 22 ± 2 | 0.4 ± 0.2 | 14 ± 0.5 | 4.1 ± 0.5 |
| F6 | 82 ± 12 | 83 ± 9 | 83 ± 2 | 26 ± 4 | 27 ± 3 | 27 ± 1 | 4.6 ± 0.9 | 7.5 ± 0.9 | 4.5 ± 0.3 |
| F7 | 70 ± 8 | 71 ± 4 | 72 ± 7 | 22 ± 2 | 23 ± 1 | 23 ± 2 | 9.0 ± 0.4 | 8.8 ± 0.7 | 8.3 ± 0.7 |
| F8 | 75 ± 6 | 73 ± 3 | 70 ± 2 | 24 ± 2 | 23 ± 1 | 23 ± 1 | 6.7 ± 0.4 | 8.2 ± 1.2 | 7.5 ± 0.9 |
| F9 | 61 ± 6 | 60 ± 5 | 60 ± 10 | 20 ± 2 | 19 ± 2 | 19 ± 3 | 1.1 ± 1.2 | 24 ± 2.3 | 8.3 ± 0.9 |