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mRNA expression levels for (A) NLRP3, (B) caspase-1, and (C) IL-1β were higher in the Modic changes group than for the control group. Typical Western blot bands from two patients with vertebra burst fracture and two patients with (D) Modic changes are shown. The band density analysis of (E) IL-1β, (F) caspase-1, and (G) NLRP3 were analyzed by Image J software and normalized to β-actin. *p < 0.05, **p < 0.01.
