Table 2.
Primer sequences used for quantitative RT-PCR
| Gene | Stream | Primer pair sequence |
|---|---|---|
| Col II | Forward | 5′-CTGGAAAAGCTGGTGAAAGG-3′ |
| Reverse | 5′-GGCCTGGATAACCTCTGTGA-3′ | |
| SOX9 | Forward | 5′-GGAATGTTTCAGCAGCCAAT-3′ |
| Reverse | 5′-TGGTGTTCTGAGAGGCACAG-3′ | |
| MMP3 | Forward | 5′-CTGGAATGGTCTTGGCTCAT-3′ |
| Reverse | 5′-CTGACTGCATCGAAGGACAA-3′ | |
| ADAMTS-5 | Forward | 5′-TACTTGGCCTCTCCCATGAC-3′ |
| Reverse | 5′-TTTGGACCAGGGCTTAGATG-3′ | |
| NLRP3 | Forward | 5′-ACAGCATTGAAGAGGAGTGGA-3′ |
| Reverse | 5′-TCGTGTGTAGCGTTTGTTGAG-3′ | |
| Caspase-1 | Forward | 5′-GAAGGACAAACCGAAGGTGAT-3′ |
| Reverse | 5′-TGGAAGAGCAGAAAGCGATAA-3′ | |
| IL-1β | Forward | 5′-CTGTCCTGCGTGTTGAAAGAT-3′ |
| Reverse | 5′-TTCTGCTTGAGAGGTGCTGAT-3′ | |
| SDHA | Forward | 5′-AGACCTAAAGCACCTGAAGACG-3′ |
| Reverse | 5′-ATCAATCCGCACCTTGTAGTCT-3′ |
RT-PCR = Reverse transcription-polymerase chain reaction; Col II = Type II collagen; MMP3 = matrix metalloproteases 3; ADAMTS-5 = a disintegrin-like and metalloprotease with thrombospondin type I motifs-5; NLRP3 = NACHT, LRR and PYD domains-containing protein, 3; SDHA = succinate dehydrogenase complex, subunit A.