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. 2016 Sep 25;33(5Part A):514–523. doi: 10.1016/j.nbt.2015.12.007

Figure 2.

Figure 2

Osteogenic differentiation of isolated mononuclear cell populations. Phase contrast images revealing alkaline phosphatase positive staining (black) upon BMP2 exposure of cell culture samples derived from the three extraction methods (described in ‘Materials and Methods’). Osteogenic differentiation was induced by lowering FBS concentration to 5% and by treating cells with BMP2 (0.3 μg/ml) for 5 days. In the control (CTRL), cells were grown in the same medium conditions, without addition of BMP2.