Table 4.
Features of the ORFs of phage SH2, identity with SH1, predicted functions of proteins, and best matches with database.
| Predicted protein | Aligned protein | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ORF | SH1 ORF,% | Start (bp) | Stop (bp) | Size (aa) | MW(kDa) | pI | SD sequence TAAGGAGGT (5′-3′)a | Predicted function | BLAST (extent; %aa identity)b | E Value | Size (aa) | Accession number |
| 1 | ORF1, 99 | 948 | 1406 | 152 | 17.0 | 7.6 | TGAGGTAACaccaaATG | S-adenosyl-l-methionine hydrolase | gp0.3 [Yersinia phage phiYeO3-12] (152/152; 100%) | 4.00E-110 | 152 | NP_052065.1 |
| 2 | ORF2, 87 | 1479 | 1679 | 66 | 7.5 | 6.8 | ATAGGACTAacaccATG | gp0.45 [Salmonella phage phiSG-JL2] (65/66; 98%) | 4.00E-41 | 66 | YP_001949746.1 | |
| 3 | ORF3, 74 | 1699 | 1857 | 52 | 6.0 | 9.7 | ACAGGAGGAttagcaATG | hypothetical protein [Enterobacter phage E-4] (49/52; 94%) | 3.00E-26 | 52 | AKA61646.1 | |
| 4 | ORF4, 94 | 1854 | 2051 | 65 | 7.8 | 10.5 | TGGTGAAACacgcATG | hypothetical protein [Enterobacter phage E-4] (63/65; 97%) | 2.00E-38 | 65 | AKA61645.1 | |
| 5 | ORF5, 95 | 2073 | 3182 | 369 | 42.3 | 7.1 | TAAGGACACactgaaATG | Protein kinase | gp0.7 [Yersinia phage phiYeO3-12] (346/369; 94%) | 0.0 | 369 | NP_052070.1 |
| 6 | ORF6,99 | 3253 | 5907 | 884 | 98.8 | 7.1 | CAATGAGGTaagcaATG | RNA polymerase | DNA-directed RNA polymerase [Enterobacter phage E-2] (881/884; 99%) | 0.0 | 884 | AKA61565.1 |
| 7 | ORF7, 90 | 6008 | 6502 | 164 | 19.6 | 9.2 | TAAGAGGATtactttATG | gp1.05 [Salmonella phage phiSG-JL2] (76/165; 46%) | 3.00E-38 | 169 | YP_001949751.1 | |
| 8 | ORF8, 100 | 6593 | 6733 | 46 | 5.9 | 10.9 | TAAGATACTATG | gp1.1 [Yersinia phage phiYeO3-12] (46/46; 100%) | 2.00E-23 | 46 | NP_052073.1 | |
| 9 | ORF9, 69 | 6736 | 7008 | 91 | 10.3 | 7.9 | AGTGGAACTaatgATG | Deoxyguanosine triphospho-hydrolase inhibitor | gp1.2 [Salmonella phage phiSG-JL2] (90/91; 99%) | 1.00E-160 | 92 | YP_001949753.1 |
| 10 | ORF10, 98 | 7103 | 8119 | 338 | 38.4 | 5.0 | TGAGGAACAaccgtATG | DNA ligase | gp1.3 [Salmonella phage phiSG-JL2] (333/338; 99%) | 0.0 | 338 | YP_001949754.1 |
| 11 | ORF11, 94 | 8291 | 8548 | 85 | 9.9 | 11.2 | TAAGGAGACaacatcATG | LysR family transcriptional regulator | gp1.6 [Yersinia phage phiYeO3-12] (85/85; 100%) | 2.00E-53 | 85 | NP_052078.1 |
| 12 | ORF12, 66 | 8548 | 9132 | 194 | 21.6 | 9.1 | TAAGGAGGTgctgtaATG | Nucleotide kinase | phiYe-F10_00014 [Yersinia phage phiYe-F10] (163/190; 86%) | 6.00E-115 | 190 | AKQ06773.1 |
| 13 | ORF14, 85 | 9119 | 9256 | 45 | 5.3 | 5.2 | TAAGGGGCTgtgctATG | AVU28_gp19 [Enterobacter phage E-3] (42/45; 93%) | 1.00E-21 | 45 | AKA61598.1 | |
| 14 | ORF15, 62 | 9253 | 9489 | 78 | 8.8 | 4.8 | TAAGGAGGCcaataaATG | Bacterial RNA polymerase inhibitor | ORF13 [Yersinia phage vB_YenP_AP5] (77/78; 99%) | 1.00E-50 | 78 | AIM40358.1 |
| 15 | ORF16, 98 | 9542 | 10240 | 232 | 26.0 | 4.8 | AAAGGAGAAacatcATG | Single-stranded DNA-binding | phiYe-F10_00017 [Yersinia phage phiYe-F10] (227/232; 98%) | 4.00E-166 | 232 | AKQ06776.1 |
| 16 | ORF18, 73 | 10240 | 10701 | 153 | 17.6 | 9.5 | CGAGGACTTctaATG | Endonuclease | gp3 [Yersinia phage phiYeO3-12] (152/153; 99%) | 2.00E-105 | 153 | NP_052083.1 |
| 17 | ORF19, 99 | 10694 | 11149 | 151 | 16.9 | 9.0 | TAAAGAAAAATG | N-acetylmuramoyl-l-alanine amidase | AVU28_gp15 [Enterobacter phage E-3] (151/151; 100%) | 2.00E-108 | 151 | AKA61594.1 |
| 18 | ORF20, 100 | 11154 | 11261 | 35 | 4.2 | 8.5 | GAGGGTGATaccATG | 3.7 protein [Yersinia phage phiYeO3-12] (35/35; 100%) | 2.00E-15 | 35 | NP_052086.1 | |
| 19 | ORF21,99 | 11328 | 13028 | 566 | 6.3 | 5.2 | TAAGGAATGtacaATG | Primase/Helicase | gp4A [Salmonella phage phiSG-JL2] (560/566; 99%) | 0.0 | 566 | YP_001949764.1 |
| 19.1 | ORF21.1, 94 | 11362 | 11517 | 51 | 5.6 | 6.5 | TCTTTCTGTttcATG | hypothetical protein [Enterobacteria phage T3] (49/51; 96%) | 1.00E-26 | 51 | AGM10719.1 | |
| 19B | ORF21B,99 | 11514 | 13028 | 504 | 55.9 | 5.1 | GGAGGCAGTaaccctATG | Primase/Helicase | Primase/Helicase protein [Enterobacter phage E-3] (501/504; 99%) | 0.0 | 504 | AKA61593.1 |
| 19.2 | ORF21.2, 97 | 12748 | 13077 | 109 | 12.1 | 6.7 | GAAGGGAAAaccacATG | gp4.2 [Enterobacteria phage T3] (103/109; 94%) | 2.00E-66 | 109 | NP_523317.1 | |
| 20 | ORF22, 97 | 13124 | 13336 | 70 | 7.7 | 10.0 | ATAGGAGACacatcATG | gp4.3 [Yersinia phage phiYeO3-12] (68/70; 97%) | 4.00E-29 | 70 | NP_052091.1 | |
| 21 | ORF23, 100 | 13349 | 13633 | 94 | 10.7 | 9.9 | TAAGGAGCGaacactATG | gp4.5 [Yersinia phage phiYeO3-12] (94/94; 100%) | 2.00E-62 | 94 | NP_052092.1 | |
| 22 | ORF24, 98 | 13701 | 15815 | 704 | 79.8 | 6.5 | AAAGGAGGGcattATG | DNA polymerase | gp5 [Yersinia phage phiYeO3-12] (700/704; 99%) | 0.0 | 704 | NP_052093.1 |
| 23 | 15825 | 16157 | 110 | 13.0 | 9.5 | TAAGGAGGAtttATG | Homing Endonuclease | gp5.3 [Yersinia phage phiYeO3-12] (110/110; 100%) | 2.00E-75 | 110 | NP_052095.1 | |
| 24 | ORF25, 99 | 16135 | 16437 | 101 | 11.1 | 6.3 | AAAGGAGAAacattATG | HNS binding | gp5.5 [Yersinia phage phiYeO3-12] (100/101; 99%) | 4.00E-66 | 101 | NP_052097.1 |
| 25 | ORF26, 100 | 16553 | 16762 | 69 | 7.3 | 9.8 | TTGGGAGGTactctaATG | gp5.7 [Yersinia phage phiYeO3-12] (69/69; 100%) | 8.00E-42 | 69 | NP_052098.1 | |
| 26 | ORF27, 99 | 16705 | 16941 | 60 | 8.8 | 4.2 | CAATGGTGGagcATG | gp5.9 [Yersinia phage phiYeO3-12] (60/60; 100%) | 1.00E-34 | 60 | NP_072071.1 | |
| 27 | ORF28, 99 | 16938 | 17849 | 303 | 34.7 | 4.9 | GGAGGATGAcgaATG | Exonuclease | AVU28_gp07 [Enterobacter phage E-3] (301/303; 99%) | 0.0 | 303 | AKA61586.1 |
| 28 | ORF29, 100 | 17831 | 17944 | 37 | 4.1 | 9.7 | CAAGGAGATttacttATG | gp6.3 [Yersinia phage phiYeO3-12] (37/37; 100%) | 1.00E-15 | 37 | NP_052102.1 | |
| 29 | ORF30, 96 | 18039 | 18284 | 81 | 9.3 | 5.9 | TTAAGAGGTgaatttATG | gp6.5 [Yersinia phage phiYeO3-12] (79/81; 98%) | 4.00E-51 | 81 | NP_052103.1 | |
| 30 | ORF31, 69 | 18289 | 18540 | 83 | 8.8 | 9.1 | ACAGGAGTAattatATG | Head | gp6.7 [Yersinia phage vB_YenP_AP5] (83/83; 100%) | 2.00E-49 | 83 | YP_009102822.1 |
| 31 | ORF32, 96 | 18568 | 18888 | 106 | 11.0 | 9.8 | TAGGGAGAAacatcATG | Host specificity protein B | gp7.3 [Salmonella phage phiSG-JL2] (105/106; 99%) | 1.00E-62 | 106 | YP_001949779.1 |
| 32 | ORF33, 99 | 18899 | 20506 | 535 | 58.6 | 4.5 | TAAGGAGGActgaATG | Head-to-tail joining | gp8 [Yersinia phage phiYeO3-12] (535/535; 100%) | 0.0 | 535 | NP_052106.1 |
| 33 | ORF34, 97 | 20608 | 21540 | 310 | 33.8 | 4.3 | TTAGGAGATttaacaATG | Capsid assembly | ORF30 [Citrobacter phage phiCFP-1] (303/310; 98%) | 0.0 | 310 | AKA62148.1 |
| 34 | ORF35, 95 | 21697 | 22740 | 347 | 36.8 | 6.2 | TAAGGAGATtcaacATG | Minor and Major capsid | 10A [Yersinia phage phiYe-F10] (344/346; 99%) | 0.0 | 347 | AKQ06793.1 |
| 35 | 22779 | 23012 | 77 | 7.4 | 4.5 | TCAGAAGACtATG | Minor capsid | AVU28_gp35 [Enterobacter phage E-3] (73/77; 95%) | 1.00E-39 | 77 | AKA61614.1 | |
| 36 | ORF36, 99 | 23125 | 23715 | 196 | 22.2 | 4.5 | ACAGGAGGTaacatcATG | Tail tubular A | gp11 [Yersinia phage phiYeO3-12] (196/196; 100%) | 1.00E-141 | 196 | NP_052110.1 |
| 37 | ORF37, 99 | 23731 | 26136 | 801 | 89.8 | 5.9 | CAAGGAGGCtctATG | Tail tubular B | gp12 [Salmonella phage phiSG-JL2] (797/801; 99%) | 0.0 | 801 | YP_001949785.1 |
| 38 | ORF38, 98 | 26209 | 26619 | 136 | 15.8 | 5.6 | TAAAGCATTATG | Internal virion A | AXI78_gp37 [Enterobacter phage E-2] (134/136; 99%) | 4.00E-95 | 136 | AKA61575.1 |
| 39 | ORF39, 100 | 26622 | 27215 | 197 | 21.2 | 9.4 | GTAGGAGGTaactATG | Internal virion B | gp14 [Yersinia phage phiYeO3-12] (194/197; 98%) | 4.00E-136 | 197 | NP_052114.1 |
| 40 | ORF40, 80 | 27218 | 29461 | 747 | 84.6 | 6.1 | CCGGGAGGTaataATG | Internal virion C | ORF37 [Citrobacter phage phiCFP-1] (711/747; 95%) | 0.0 | 747 | AKA62155.1 |
| 41 | ORF41, 83 | 29484 | 33452 | 1322 | 144.2 | 6.7 | TAAGGAGGCtccATG | Internal virion D | ORF38 [Citrobacter phage phiCFP-1] (1296/1322; 98%) | 0.0 | 1322 | AKA62156.1 |
| 42 | ORF42, 92 | 33524 | 35500 | 658 | 69.9 | 6.0 | AAAGGAGGTcacATG | Tail fiber | gp17 [Salmonella phage phiSG-JL2] (593/658; 90%) | 0.0 | 658 | YP_001949790.1 |
| 43 | ORF43, 99 | 35511 | 35714 | 67 | 7.4 | 6.1 | TAAGGAGGAcataATG | Lysis | gp17.5 [Yersinia phage phiYeO3-12] (66/67; 99%) | 5.00E-39 | 67 | NP_052118.1 |
| 44 | ORF44, 100 | 35718 | 35984 | 88 | 9.9 | 4.7 | CAAGGAGTAacctATG | DNA packaging A | gp18 [Salmonella phage phiSG-JL2] (88/88; 100%) | 1.00E-55 | 88 | YP_001949792.1 |
| 45 | ORF45, 99 | 36062 | 36526 | 150 | 17.3 | 9.2 | ATGGGAGGTgttATG | Endopeptidase Rz | ORF42 [Citrobacter phage phiCFP-1] (152/154; 99%) | 1.00E-107 | 154 | AKA62160.1 |
| 45.7 | ORF45.7, 99 | 36189 | 36443 | 84 | 9.3 | 9.8 | TAATCCAAAATG | gp18.7 [Salmonella phage phiSG-JL2] (83/84; 99%) | 9.00E-52 | 84 | YP_001949794.1 | |
| 46 | ORF46, 99 | 36501 | 38264 | 587 | 66.6 | 5.3 | TAAGGAGATgcagaATG | DNA packaging B | gp19 [Salmonella phage phiSG-JL2] (581/587; 99%) | 0.0 | 587 | YP_001949795.1 |
| 46.2 | ORF46.2, 93 | 37213 | 37383 | 56 | 6.1 | 10.0 | GAAGACTTGtactATG | 19.2 protein [Yersinia phage phiYeO3-12] (56/56; 100%) | 2.00E-28 | 77 | NP_052123.1 | |
| 46.3 | ORF46.3, 95 | 37687 | 37815 | 42 | 4.7 | 11.9 | TGGCGGGTTccgcgATG | 19.3 protein [Yersinia phage phiYeO3-12] (42/42; 100%) | 1.00E-19 | 42 | NP_052124.1 | |
| 47 | ORF47, 96 | 38509 | 38658 | 49 | 5.5 | 7.9 | AAAGGAGGTggctcAATG | AVU28_gp23 [Enterobacter phage E-3] (48/49; 98%) | 9.00E-25 | 49 | AKA61602.1 | |
a
Start codon indicated in boldface; Match to SD sequence is indicated by underlining; SD position is indicated in uppercase.
b
The number of identical amino acids/The total number of amino acids of smallest protein.