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. 2016 Jun 29;7:82. doi: 10.3389/fendo.2016.00082

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Copyright © 2016 Bell and Murray.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gap junction (Cx43) localization and dye communication in the intact adrenal gland. Immunohistochemical localization of Cx43 gap junction proteins revealed extensive staining in the zona fasciculata (ZF) and zona reticularis (ZR), while there was limited staining in the zona glomerulosa (ZF) [left panels: (A,C)]. Correspondingly, lucifer yellow dye communication between cells was more abundant in the inner zones of the adrenal cortex (ACTH responsive areas) than in the outer zone [right panels: (B,D)]. Capsule (arrowheads), connective tissue trabecule (arrow), and Cx43 (white puncta). Bars: (A,B) 50 μm and (C,D) 30 μm [modified from Ref. (30)].