Figure 5.

Cocultured adrenal/granulosa cell pairs. Adrenal cell clusters viewed with optics specific for fluorescein isothiocyanate (A) or rhodamine isothiocyanate (B) were treated with follicle-stimulating hormone (FSH) for 30 min. Abundant protein kinase dissociation was initially observed in granulosa cells (OG) and could be seen, after 5–15 min, in two adrenal cells (Y-1*) that are in contact with the granulosa cell, while two nearby adrenal cells (Y-1) that are not in contact with the granulosa cell failed to dissociate PKA (A). Granulosa cells were identified by pre-labeling them with rhodamine-coated beads (B) [modified from Ref. (59)].