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. 2016 Apr 10;8(4):796–808. doi: 10.18632/aging.100943

Figure 1. Identification and validation of small open reading frames (sORFs) within the mitochondrial 16S ribosomal RNA (rRNA) gene.

Figure 1

(A) Assigned names, size, location, and predicted sequences of SHLPs. (B) Location of SHLP ORFs within 16S rRNA. (C) Relative expression of SHLPs (1–4 and 6) in C57BL/6 mouse tissues relative to GAPDH (used as a loading control). (D) Expression of SHLPs (1–4 and 6), mitochondrial complexes (COI and COII) and nuclear GAPDH proteins in HeLa parental and HeLa-ρ0 cells. (E) Northern blot of whole cell RNA, whole cell mRNA, mitochondrial RNA, and mitochondrial mRNA using a SHLP6 probe identifies several bands smaller than the 16S rRNA indicated by the arrows.