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. 2016 Apr 10;8(4):796–808. doi: 10.18632/aging.100943

Figure 3. SHLP2 and SHLP3 modulate mitochondrial function.

Figure 3

The effects of exogenous SHLP2 and SHLP3 on mitochondria were assessed in 22Rv1 cells incubated with 100 nM control peptide, SHLP2, or SHLP3 for 24 h by measuring (A) oxygen consumption rate (OCR) performed on a Seahorse XF24 Extracellular Flux Analyzer and (B) ATP production. (C) Reactive oxygen species (ROS) production as assessed by DHE (dihydroethidium) fluorescence in NIT-1 (top) and 22RV1 (bottom) cells after incubation with 100 nM control peptide, SHLP2, or SHLP3 overnight. All data are presented as means ± SEM. (D) NIT-1 β cells were pre-incubated with 100 nM SHLP2 or SHLP3 for 5 h, followed by incubation with 10 μM staurosporine (STS) for 24 h. Apoptosis (pre-G1 peak) was assessed by FACS (fluorescence-activated cell sorting) analysis. *P < 0.05; **P < 0.01; ***P < 0.001.