Figure 6. Circulating SHLP2 levels decrease with age in mice and protect against amyloid-beta (Aβ) toxicity.

(A) Plasma SHLP2 levels in young and old male and female C57BL/6 mice (n = 10 in each group) were assessed by ELISA. (B) Mouse primary cortical neurons were treated with vehicle control, 10 μM Aβ_1–42 alone, or co-treated 10 μM Aβ_1–42 with 1 nM, 100 nM, or 10 μM SHLP2. Aβ_1–42-induced cytotoxicity was measured using a LDH (lactate dehydrogenase) cytotoxicity assay kit.