Figure 3.

Effect of 10 and 100 μg/mL Citrus bergamia extract (CBE) on the lipid content of 14-day differentiated adipocytes stained with oil red O. (a) A representative photograph from three independent experiments of untreated mesenchymal stem cells (Control), 14-day differentiated cells (adipocytes), adipocytes with 10 or 100 μg/mL CBE at 7 and 14 days was shown. (b) The levels of oil red O incorporation was quantified by measuring the absorbance of isopropyl alcohol extract at λ = 550 nm. Data were shown relative to 14-day differentiated adipocytes and expressed as mean ± SD from three independent experiments. ^∗ p < 0.01 versus the 14-day differentiated adipocytes (100%) at same day; °p < 0.01 versus CBE 10 μg/mL at 7 days; ^• p < 0.01 versus CBE 100 μg/mL at 7 days.