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. 2016 Jun 15;2016:4563815. doi: 10.1155/2016/4563815

Figure 4.

Figure 4

Inhibitory effects of 10 or 100 μg/mL Citrus bergamia extract (CBE) on adipocyte differentiation in mesenchymal stem cells induced to differentiate into adipocytes by using adipogenic medium in presence or absence of CBE. At 7, 14, 21, and 28 days, differentiated mesenchymal stem cells adipocytes were examined for PPAR-γ, A-FAB, AMPKα1/2, and p-AMPKα1/2 by Western blot. The levels of proteins were expressed as arbitrary densitometric units (ADU) and the ratio p-AMPKα1/2/AMPKα1/2. Data are shown relative to adipogenic-treated cells and expressed as mean ± SD from three independent experiments. ∗ p < 0.01 versus the mesenchymal stem cells treated with adipogenic medium at same day; °p < 0.01 versus CBE 10 μg/mL at 28 days; • p < 0.01 versus CBE 100 μg/mL at 28 days.