Fig. 2.
Gene expression of runt-related transcription factor 2, alkaline phosphatase, osteocalcin and osteopontin at day 4 (A), alkaline phosphatase protein expression at day 17 (B), and extracellular matrix mineralization at day 21 (C) of hMSCs differentiated into osteoblasts and cultured on nanotopography and control Ti surfaces. The data are presented as mean ± standard deviation (n=3 for gene expression and n=5 for extracellular matrix mineralization). * Indicates statistically significant difference (p≤0.05).