Table 1.
Step | Total Protein (mg) | Total Activity (U) | Specific Activity * (U/mg) | Folds | Recovery (%) |
---|---|---|---|---|---|
Crude enzyme | 1331 | 100,548 | 76 | 1 | 100 |
(NH4)2SO4 precipitation | 73.2 | 37,625 | 514 | 6.8 | 37.4 |
SephadexG-200-filtration | 0.73 | 9577 | 13,042 | 172.6 | 9.5 |
SephadexG-75-filtration | 0.28 | 6770 | 24,083 | 318.1 | 6.7 |
* Note: One unit was defined as the amount of enzyme required to increase the absorbance at 235 nm by 0.01 per min.