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. 2016 Jun 29;6:28882. doi: 10.1038/srep28882

Figure 10. Recombinant OPN stimulates ERK signaling in primary mesangial cells.

Figure 10

(A) Immunoblot for phosphorylated ERK and total ERK. Primary mesangial cells were incubated with rmOPN (0 ng/ml, 300 ng/mL) for up to 30 min. Bar graph shows quantification of phosphorylated ERK and total ERK expression. (B) Immunoblot for phosphorylated ERK and total ERK following pre-treatment with an ERK inhibitor. Primary mesangial cells were grown in culture and pre-incubated with an inhibitor of ERK (PD98059; 20 μmol/L) for 30 min followed by treatment with rmOPN for 30 min. Bar graph shows quantification of phosphorylated ERK and total ERK expression. (C) Immunoblot for LOX-1. Primary mesangial cells were pre-incubated with an inhibitor of ERK (PD98059; 20 μmol/L) for 30 min followed by treatment with rmOPN for 48 h. Bar graph shows quantification of LOX-1 protein expression. RmOPN induced LOX-1 protein expression in primary mesangial cells. Data are means ± SEM; n = 3 in each group. Difference between two groups was evaluated by Student’s t-test for unpaired variables and differences among three and four groups were analyzed by one-way ANOVA and subsequent Tukey’s test. *P < 0.05; **P < 0.01.