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. 2016 Jun 29;6:28882. doi: 10.1038/srep28882

Figure 9. Recombinant mouse OPN(rmOPN) induces LOX-1 expression in primary mesangial cells.

Figure 9

(A) Relative mRNA expression of LOX-1 in primary mesangial cells. Primary mesangial cells were incubated with rmOPN (0 ng/mL, 150 ng/mL, 300 ng/mL) for up to 24 h. (B) Immunoblotting for LOX-1 protein in primary mesangial cells. Primary mesangial cells were incubated with or without rmOPN (0 ng/mL, 300 ng/mL) for up to 48 h. (C) Bar graph shows quantification of LOX-1 protein expression. Data are means ± SEM; n = 4–6 in each group. Difference between two groups was evaluated by Student’s t-test for unpaired variables and differences among three groups were analyzed by one-way ANOVA and subsequent Tukey’s test. *P < 0.05.