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The GSH-NEM conjugate was analyzed by reverse-phase HPLC with ultraviolet detection at 265 nm (r.t. 4.36 min). GSH depletion in erythrocytes treated with DMSO (0.2% v/v), Bay 11–7082 (20 μM), parthenolide (50 μM) or dimethyl fumarate (DMF: 140 μM) for 24 h are shown.
