Figure 1.

(a) Elution profile of gel-filtration chromatography on a Sephacryl S-200 HR column of Rituximab/saporin-S6 conjugate. The blue line represents the A₂₈₀, and the red dashed line represents the radioactivity of the eluted fractions. The fractions corresponding to the peaks were pooled and indicated with capital letters: (A) high-molecular-weight immunotoxin (HMW-IT) (blue area); (B) low-molecular-weight immunotoxin (LMW-IT) (green area); (C) free Rituximab; (D) trimmers; (E) dimers and (F) monomers of saporin-S6. (b) Analysis of fractions corresponding to HMW-IT (1), LMW-IT (2), and unconjugated Rituximab (3) by SDS–PAGE under non-reducing conditions on a 4%–15% PhastGel. Standard molecular weights (St) are expressed in kDa.