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. 2016 Jun 29;6:28892. doi: 10.1038/srep28892

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Copyright © 2016, Macmillan Publishers Limited

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Results of NAT assays with substrate peptides DP8, DP9, DP10 and DP11 analyzed using MALDI-TOF/TOF. (a) MS/MS of 910.5 Da precursor ion (S/N = 4201) of unmodified and 952.5 Da precursor ion (S/N = 5894) of modified substrate peptide representing novel substrate specificity of RimI^Mtb (b) MS/MS of 999.5 Da precursor ion (S/N = 7037) of unmodified and 1041.5 Da precursor ion (S/N = 4256) of modified substrate peptide representing N-terminus of conventional NatE substrate (c) MS/MS of 1057.8 Da precursor ion (S/N = 15916) of unmodified and 1099.5 Da precursor ion (S/N = 1011) of modified substrate peptide representing N-terminus of conventional NatB substrate (d) MS/MS of 1041.5 Da precursor ion (S/N=17767) of unmodified and 1083.5 Da precursor ion (S/N = 14629) of modified substrate peptide representing N-terminus of conventional NatC substrate.