Figure 2.

Real-time polymerase chain reaction (RT-PCR) analysis of transformed Vero cells. The presence of a 130 bp fragment (t-RNA^val promoter + ribozyme) for each ribozyme (A) demonstrates stable transformation of the clonal cells. The absence of this 130 bp fragment in the RT-negative control (B) indicates a lack of genomic DNA contamination in the isolated RNA. The low molecular weight smears apparent in each lane are attributed to primer dimers as they are well below the lowest size standard in the gel. The forward primer used was specific to the common t-RNA^val promoter, and the reverse primer was specific for each ribozyme. M = 1 kb plus DNA ladder, lanes 9–15 correspond to hRzs #9 through #15.