Table 1.
Template sequences used to amplify the t-RNAval Pol III + hRz + PolyA(60) expression cassettes for each hRz, and corresponding CHIKV target sites. The hRzs’ sequence is indicated in uppercase.
| hRz | Template Sequence | Target Sequence (5’–3’) |
|---|---|---|
| hRz#9 | cgaacccgggcactacaaaaaccaacaaCTATTTAGCTGATGAGGCCGAAAGGCCGAAACCGCCGTACgggcccgggcccaaaaa | gtacggcggtcctaaatag |
| hRz#10 | cgaacccgggcactacaaaaaccaacaaAAGCGTCGCTGATGAGGCCGAAAGGCCGAAACTTCATGTGggggcccgggcccaaaaa | cacatgaagtccgacgctt |
| hRz#11 | cgaacccgggcactacaaaaaccaacaaTTACGCGGCTGATGAGGCCGAAAGGCCGAAACCAGAGGGgggcccgggcccaaaaa | ccctctggtcccgcgtaa |
| hRz#12 | cgaacccgggcactacaaaaaccaacaaAGCATGATCTGATGAGGCCGAAAGGCCGAAACTTGGTTTTggggcccgggcccaaaaa | aaaaccaagtcatcatgct |
| hRz#13 | cgaacccgggcactacaaaaaccaacaaAATGGGTACTGATGAGGCCGAAAGGCCGAAACGCCGGTGAgggcccgggcccaaaaa | tcaccggcgtctacccatt |
| hRz#14 | cgaacccgggcactacaaaaaccaacaaAGGCTGAACTGATGAGGCCGAAAGGCCGAAACATTGGCCCgggcccgggcccaaaaa | gggccaatgtcttcagcct |
| hRz#15 | cgaacccgggcactacaaaaaccaacaaTCTTAGGGCTGATGAGGCCGAAAGGCCGAAACACATATACgggcccgggcccaaaaa | gtatatgtgtcccctaaga |