Figure 3. Impact of gangliosides and cholesterol levels for amyloid pore formation.
Effect of 1 mM methyl-β-cyclodextrin (mβCD) pretreatment on amyloid pores induced by 220 nM Aβ1-42 or Aβ22-35 (A), or 220 nM α-synuclein1-140 or α-synuclein67-78 (B). Effect of PPMP pretreatment on amyloid pores induced by Aβ1-42 and Aβ22-35 (C) or α-synuclein1-140 or α-synuclein67-78 (D) (***p < 10−9 with 59 < n < 170). The cells were washed 3 times after mβCD or PPMP treatment, and then again 3 times after the incubation with the Fluo-4AM probe. Under these conditions, mβCD and PPMP were totally removed at the time the amyloid proteins were added to the cells (Supplementary Fig. S2).