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. 2016 Jun 29;6:28156. doi: 10.1038/srep28156

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Copyright © 2016, Macmillan Publishers Limited

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Occurrences of fluorescence emission with respect to beam illumination duration was measured at different illumination beam fluences from (a) air and (b) water-immersed PMMA films. We continuously measured the number of total fluorescence emission events within each 100-ms integration time for 30 seconds. The occurrence from the air sample dropped significantly faster than from the water-immersed sample. The initial decay stage lasts for about 30 seconds, but is most prominent in the first 5 seconds. We then observed stable blinking at an average frequency of 0.013 events per second at 2 kW/cm² and 0.582 events per second at 2 kW/cm² per square micrometer from the air and water-immersed PMMA film samples, respectively.