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. 2016 Jun 29;6:29039. doi: 10.1038/srep29039

Figure 4. Validation of ssc-chimeric-113.

Figure 4

(A) Detection of ssc-chimeric-113 via transcriptome sequencing. The “grep” command was used to identify 37 reads spanning the exon-junction. (B) Validation of ssc-chimeric-113 by PCR amplification and electrophoresis. Lane 1: marker. Lane 2: electrophoresis result. Lane 3: no template control. (C) Validation of the ssc-chimeric-113 breakpoint using Sanger sequencing. (D) BLAT of the Sanger sequencing result on Sus scrofa genome assembly 10.2 (http://genome.ucsc.edu/cgi-bin/hgBlat).