Figure 3. DNA sequence upstream of other isoforms is conserved among species.

We performed global alignment on promoter sequences (upstream of start codon) between orthologous genes, and used the resulting alignment score as sequence conservation score. Average values that correspond to sequence conservation score are shown for gene promoters covered by isoform promoters (150 bp upstream) and those not covered by isoform promoters. As [−100, 0] in all gene promoters are covered by isoform promoters, we excluded this region for analysis. Promoter is divided into three bins. (A) Orthologous genes between S. cerevisiae and S. mikatae. (B) Orthologous genes between S. cerevisiae and S. kudriavzevii. Error bars were calculated by bootstrapping. The statistical significant values calculated from Mann-Whitney U-test were indicated.