Fig. 7.

Western blot and immunocytochemical analyses of nuclear factor-kappaB (NF-κB) in the nucleus and cytoplasm of human corneal fibroblasts (HCFs) with or without angiogenin (ANG) treatment. a After ANG treatment, nuclear translocation of NF-κB induced by tumor necrosis factor-alpha (TNF-α) or lipopolysaccharide (LPS) was decreased. b Densitometric analysis of the nuclear expression of NF-κB protein relative to lamin in HCFs. The experiments were performed in triplicate. One-way ANOVA followed by Bonferroni’s post hoc analysis, *p < 0.05. c Immunocytochemistry of NF-κB in the nuclei and cytoplasm of HCFs. The intranuclear fluorescence of NF-κB induced by TNF-α or LPS was suppressed by ANG treatment. Scale bar, 100 μm