Table 1.
Sequences of PCR primers
| Gene | Sense primer (5’→3’) | Anti-sense primer (3’→5’) | Product size (bp) |
|---|---|---|---|
| GAPDH | CGAGATCCCTCCAAAATCAA | TGTGGTCATGAGTCCTTCCA | 294 |
| IL-1β | CCTGTCCTGCGTGTTGAAAGA | GGGAACTGGGCAGACTCAAA | 150 |
| IL-4 | AACACAACTGAGAAGGAAACCTTC | GCTCGAACACTTTGAATATTTCTC | 276 |
| IL-6 | TTCGGTCCAGTTGCCTTCTC | GAGGTGAGTGGCTCTCTGTG | 112 |
| IL-8 | ACATGACTTCCAAGCTGGCCG | TTTATGAATTCTCAGCCCTC | 302 |
| IL-10 | GCCTAACATGCTTCGAGATC | TGATGTCTGGGTCTTGGTTC | 206 |
| TLR4 | AGCCTAAGCCACCTCTCTACCT | AGATTTGTCTCCACAGCCACCA | 116 |
| MYD88 | ATGGTGGTGGTTGTCTCTGATG | GACAGGATGAACCTCAGGATGC | 165 |
| TNFR1 | GTGCTGTTGCCCCTGGTCAT | GCTTAGTAGTAGTTCCTTCA | 163 |
| TNFR2 | AAACTCAAGCCTGCACTC | GGATGAAGTCGTGTTGGAGA | 209 |
| C1r | CAACAACTTTGAAACAACCA | GGAGAAGTCTGTGTGGAAGG | 237 |
| C1s | AAGTCAGACTTTTCCAATGA | ACTTGCAATCTCCCCAATCA | 247 |
| C2 | TGGAGTGGACAAGCTGTGCCG | TGAAAGTCTCGTGGCGGCGG | 289 |
| C3 | TGGCTGTTCGCACCCTGGAT | AGCCCGAGGGGGTCACAATGA | 204 |
| C4 | ATGGTTCCTATGCGGCTTGGTTGTC | GCGATGGTCACAAAGGCTGTGAGTG | 256 |
| C5 | TGGCAACCAGCTCCAGGTTCAT | TGCCCCACAGCCCAGATCACT | 208 |
| CFB | CAAGAAGGCCCTCCAGGCAGT | AAGGCCCGACCCCAAACACAT | 233 |
| TBK1 | TTCTGGAAGTCCATACGCAT | ACTGGTGATCTCTATGCTGT | 237 |
| IKK-ε | CTGCTCATGAATGACAGTGA | GGCGAGTGTATGTTATGCTT | 132 |