Table 2.
Physiological parameters in diabetic and non-diabetic mice.
| Gene Target | Gene Bank Accession Number | Probe 5′-3′ | Forward Primer 5′-3′ | Reverse Primer 5′-3′ |
|---|---|---|---|---|
| Advanced glycosylation end product specific receptor – (RAGE) | NM_007425 | 6-FAM CACAGCCCGGATTG |
GCTGTAGCTGGTGGTCAGAACA | CCCCTTACAGCTTAGCACAAGTG |
| Advanced glycation end products - receptor 1 (AGER1) | D89063 | 6- FAM ATCAACGTGGAGACCAT |
CCCGTCGGTGGAAGATTTT | GCTGCCGCCACCATCA |
| Advanced glycation end products-receptor 3 (AGER3) | X16834 | 6- FAM CTGGCTCTGGAAACC |
CAGACAGCTTTTCGCTTAACGA | CCATGCACCCGGATATCC |
| Angiotensinogen | NM_007428 | 6- FAM CCTCCTCGAACTCAAA |
AGTGGGAGAGGTTCTCAATAGCA | GACGTGGTCGGCTGTTCCT |
| Angiotensin II Type 1a Receptor (AT1a) | NM_177322 | 6-FAM TCTCGCCTCCGCCG |
CCATTGTCCACCCGATGAA | TGACTTTGGCCACCAGCAT |
| Angiotensin II Type 2 Receptor – (AT-2) | BC003811 | 6-FAM AATCGGTCATCTACCC |
ATTACCTGCATGAGTGTCGATAGG | GGGATTCCTTCTTTGAGACAGAAA |
| Renin | NM_031192 | 6-FAM TTCGCCTTGGCCCGC |
GGCATAACAATCGCATTGGA | GGACAAAGCCAGACAAAATGG |
Data was collected at the end of the study and is presented as Mean ± SD. WT – Wild Type, WT D – Wild Type Diabetic, RAGE KO – RAGE Knockout mice, AT-2 KO – Angiotensin II receptor type 2 Knockout mice.
*
p <0.05 vs WT,
†
p <0.05 vs RAGE KO,
‡
p <0.05 vs AT-2 KO,
§
p <0.05 vs WT D,
||
p <0.05 vs RAGE KO D.