Figure 4.

Regulation of leptin-AI and leptin-AII IP injection on orexigenic and anorexigenic factors expressed in selected brain regions in goldfish. The brain regions include the telencephalon (A), hypothalamus (B), optic tectum (C) and cerebellum (D). The detected orexigenic factors included NPY, AgRP, orexin and apelin, and the anorexigenic factors included CART, CCK, MCH and POMC. In this study, IP injection with FFPS was used as the control treatment, and real-time PCR of β-actin was used as the internal control. The data (n = 10) were expressed as a percentage of the control group. Data are expressed as the mean ± SE, and significant differences between treated and untreated groups were assessed by Student’s t-test (* p < 0.05, ** p < 0.01 and *** p < 0.001).