Table 1.

Primers and amplification conditions for PCR analysis in this study.

Gene Target/Accession No. (Primer Sequences, 5′-3′)	PCR Condition				Cycle	Tm	Product Size
	Denaturing	Annealing	Extension	Detection
NPY/M87297
GTAGTGTTGCGGGTAGCGA	94 °C	64 °C	72 °C	88 °C	×35	92 °C	234 bp
CAGACACCCCGACCCAAG	30 s	30 s	30 s	20 s
AgRP/AJ555492
TGGCATCACATCCAAACCT	94 °C	64 °C	72 °C	82 °C	×35	88 °C	230 bp
CAGGTGATGACCCAAGCAG	30 s	30 s	30 s	20 s
Orexin/DQ923590
GCAGAGCTGCTCATTGTTGACGTT	94 °C	64 °C	72 °C	84 °C	×35	82 °C	286 bp
AACCTTGTGATTACCTCAGGAGT	30 s	30 s	30 s	20 s
Apelin/FJ755698
GAGCATAGCAAAGAGCTGGA	94 °C	64 °C	72 °C	89 °C	×35	94 °C	340 bp
GCTGAGGATGAGTGGCTTGT	30 s	30 s	30 s	20 s
CART/AF288810
CCAAAGGACCCGAATCTGA	94 °C	64 °C	72 °C	82 °C	×35	90 °C	171 bp
TTTGCCGATTCTTGACCCT	30 s	30 s	30 s	20 s
CCK/CAU70865
CCGCAGTCTCAGAAGATGGG	94 °C	64 °C	72 °C	87 °C	×35	91 °C	197 bp
GGAGGGGCTTCTGCGATA	30 s	30 s	30 s	20 s
MCH/AM403730
AGGCTTGAGCGAGAACTTGG	94 °C	64 °C	72 °C	86 °C	×35	91 °C	272 bp
CCCAGAAGACCTACACCTCCC	30 s	30 s	30 s	20 s
POMC/AJ431209
AAGCGCTCCTACTCCATGGA	94 °C	60 °C	72 °C	83 °C	×35	85 °C	282 bp
CTCGTCCCAGGACTTCATGAA	30 s	30 s	30 s	20 s
β-actin/AB039726
CTGGTATCGTGATGGACTCT	94 °C	56 °C	72 °C	87 °C	×35	91 °C	285 bp
AGCTCATAGCTCTTCTCCAG	30 s	30 s	30 s	20 s