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. 2016 May 24;17(6):800. doi: 10.3390/ijms17060800

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Sca-1+/CD31− CSCs^hTERT CM protects HL-1 cardiomyocytes from CoCl₂-induced hypoxic injury. Sca-1+/CD31− CSC^hTERT lysate (A) and Sca-1+/CD31− CSCs^hTERT CM (B) were subjected to a mouse cytokine antibody array detecting 21 cytokines in duplicate. 1. EGF; 2. TGF-β1; 3. HGF; 4. IGF-1; 5. IGF-2; 6. MCP-1; 7. VEGF; 8. SDF-1; 9. bFGF; 10. E-Cadherin; 11. HGF R; 12. IFN-γ; 13. IL-10; 14. TNF-α; 15. IL-6; 16. IL-1α; 17. IL-1β; 18. IL-1ra; 19. Leptin; 20. CT-1; and 21. MCP-5. Solid-lined boxes indicate dominant paracrine factors expressed in Sca-1+/CD31− CSCs^hTERT lysate or Sca-1+/CD31− CSCs^hTERT CM; (C) relative quantification of cytokine levels expressed in Sca-1+/CD31− CSCs^hTERT lysate and Sca-1+/CD31− CSCs^hTERT CM; and (D) viable cells were counted using a hemocytometer after staining with 0.2% trypan blue Sca-1+/CD31− CSCs^hTERT CM in HL-1 cardiomyocytes treated with or without 150 μM CoCl₂ for 24 h. Data represent mean ± SD from three independent experiments (** p < 0.01).

Sca-1+/CD31− CSCs^hTERT CM protects HL-1 cardiomyocytes from CoCl₂-induced hypoxic injury. Sca-1+/CD31− CSC^hTERT lysate (A) and Sca-1+/CD31− CSCs^hTERT CM (B) were subjected to a mouse cytokine antibody array detecting 21 cytokines in duplicate. 1. EGF; 2. TGF-β1; 3. HGF; 4. IGF-1; 5. IGF-2; 6. MCP-1; 7. VEGF; 8. SDF-1; 9. bFGF; 10. E-Cadherin; 11. HGF R; 12. IFN-γ; 13. IL-10; 14. TNF-α; 15. IL-6; 16. IL-1α; 17. IL-1β; 18. IL-1ra; 19. Leptin; 20. CT-1; and 21. MCP-5. Solid-lined boxes indicate dominant paracrine factors expressed in Sca-1+/CD31− CSCs^hTERT lysate or Sca-1+/CD31− CSCs^hTERT CM; (C) relative quantification of cytokine levels expressed in Sca-1+/CD31− CSCs^hTERT lysate and Sca-1+/CD31− CSCs^hTERT CM; and (D) viable cells were counted using a hemocytometer after staining with 0.2% trypan blue Sca-1+/CD31− CSCs^hTERT CM in HL-1 cardiomyocytes treated with or without 150 μM CoCl₂ for 24 h. Data represent mean ± SD from three independent experiments (** p < 0.01).