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. 2016 May 24;17(6):801. doi: 10.3390/ijms17060801

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell synchronization was induced by nutrient deprivation followed by nutrient repletion, which induced a temporal rhythm that showed an increase of autophagy LC3B by 77.9% in young fibroblasts, whereas this effect was absent in aged fibroblasts. LC3B expression was determined by RT-PCR and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) housekeeping genes (n = 3). In addition, temporal rhythm was followed over a 24 h period through fluorescent imaging specific to LC3B, showing the higher fluorescence at night versus day in young human fibroblasts but lost in aging human fibroblasts. Used with permission, Journal of Cosmetic Science, Pernodet et al. [65].

Cell synchronization was induced by nutrient deprivation followed by nutrient repletion, which induced a temporal rhythm that showed an increase of autophagy LC3B by 77.9% in young fibroblasts, whereas this effect was absent in aged fibroblasts. LC3B expression was determined by RT-PCR and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) housekeeping genes (n = 3). In addition, temporal rhythm was followed over a 24 h period through fluorescent imaging specific to LC3B, showing the higher fluorescence at night versus day in young human fibroblasts but lost in aging human fibroblasts. Used with permission, Journal of Cosmetic Science, Pernodet et al. [65].