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. 2016 Jun 2;17(6):866. doi: 10.3390/ijms17060866

Table 2.

Over-expressed proteins in CP-treated leaves after 2D electrophoresis and MALDI-ToF MS analysis.

Spot No. ID Protein Name MASCOT Search Results * Theoretical pI/Mr (kDa) Function Localization GO Biological Process
1 2 3 4
1 Q9FXH6 cyclic nucleotide-gated ion channel 8 11 17 81 9.15/86.7 Metabolism Plasma membrane Ion transport Trasmembrane potential
2 Q9M5K3 Dhydrolipoyl Dehydrogenase 16 48 230 7.0/54.2 Defense Mitochondrion Cell redox homeostasis Response to cadmium
3 P56757 ATP synthase subunit alpha 13 29 143 5.2/55.3 Energy Chloroplast ATP hydrolysis and synthesis
4 P25819 Catalase-2 20 50 275 6.6/57.2 Defense Mitochondrion/Peroxisome Cell redox homeostasis Response to oxidative stress
5 Q9FLS9 Pentatricopeptide repeat-At5g61800 16 35 155 8.7/56.7 Defense Mitochondrion Defense responses to oligogalatturonides
6 Q9S7E9 Glutamate—glyoxylate aminotransfe-rase 2 15 41 190 6.27/53.9 Metabolism Peroxisome Biosynthetic process l-alanine catabolic process
8 Q9SCX9 Glycerol-3-phosphate dehydrogenase 10 28 108 8.2/44.3 Metabolism/Defense Chloroplast Carbohydrate metabolism Glycerol 3-phosphate catabolism
9 F4JDF3 Dirigent protein 8 7 59 104 9.76/18.9 Metabolism/Defense Apoplast Phenylpropanoid biosynthetic process
10 P25697 Phosphoribulokinase 7 31 104 5.7/44.7 Metabolism/Defense Chloroplast Response to bacterium Pyrimidine salvage Pentose phosphate cycle
11 O65396 Aminomethyltransferase 14 41 174 8.5/44.7 Metabolism/Defense Mitochondrion Glycine catabolic process Response to cadmium
16 Q05431 l-ascorbate peroxidase 1 13 61 167 5.7/27.8 Defense Cytoplasm Response to oxidative stress
19 Q9SIT7 Pentatricopepti de repeat-At2g13600 5 10 61 5.6/79.1 Unknown Mitochondrion Mitochondrial mRNA modification
22 P42760 Glutathione S-transferase F6 13 85 205 5.8/23.4 Defense Cell wall/Cytoplasm Glutathione catabolic process Response to bacterium Response to abiotic stress
29 Q9C9C9 Cytosolic sulfotransferase 18 9 29 92 5.5/40.2 Metabolism/Defense Cytoplasm Glucosinolate biosynthetic process
34 O80929 60S ribosomal protein L36-1 5 37 72 11.7/12.7 Metabolism Ribosome Structural constituent of ribosome
36 P35614 Peptide chain release factor subunit 1–3 7 24 87 5.4/49.1 Metabolism Cytoplasm Protein biosynthesis
37 Q9AV97 2-dehydro-3-deoxyphosphooctonate aldolase 12 55 176 6.3/31.9 Metabolism Cytoplasm Pollen tube development and growth
38 Q9LK72 Lectin-like protein At3g16530 12 55 151 7.0/30.5 Defense Apoplast Defense response to fungus Response to chitin
39 Q9S789 Probable inactive cytidine deaminase 9 7 25 89 8.1/33.1 Metabolism Cytoplasm Cytidine deamination Pyrimidine savage
40 Q39239 Thioredoxin H4 5 34 63 5.3/13.2 Defense Cytoplasm Cell redox homeostasis Response to oxidative stress
43 Q9LJG3 GDSL esterase/lipase ESM1 12 47 158 7.6/44.4 Defense Peroxisome/secreted Responses to bacterium glucosinolate catabolism response to cold
46 Q9LUV2 Probable protein Pop3 5 73 90 5.4/12.2 Defense Cytoplasm/Plasma membrane Defense response to fungus Defense response to fungus
47 Q9C5C2 Myrosinase 2 25 51 284 7.1/63.3 Metabolism/Defense Apoplast Defense response to insect glucosinolate catabolism response to abscisic acid
48 Q9C5R8 2-Cys peroxiredoxin BAS1-like 12 51 200 5.5/29.9 Defense Chloroplast Cellular oxidant detoxification Responses to bacterium response to cold
49 Q9SA52 Chloroplast stem-loop binding protein of 41 kDa 17 52 172 242 8.2/42.7 Metabolism/Defense Chloroplast Polysaccharide metabolism Defense response to bacterium Response to abiotic stress
Q9LPW0 Glyceraldehyde-3-phosphate dehydrogenase GAPA2 13 39 120 8.2/43.1 Metabolism Chloroplast Glucose metabolic process Reductive pentose phosphate cycle
50 Q9SA52 Chloroplast stem-loop binding protein 12 44 124 8.2/42.7 Metabolism Chloroplast Photosynthesis Polysaccharide metabolic process
51 Q9SA52 Chloroplast stem-loop binding protein 11 32 113 140 8.2/42.7 Metabolism Chloroplast Photosynthesis Polysaccharide metabolic process
P25858 Glyceraldehyde-3-phosphate dehydrogenase GAPC1 7 27 65 6.6/37.0 Metabolism/Defense Cytoplasm Carbohydrate metabolism Response to redox state Response to abiotic stress
52 Q9LJG3 GDSL esterase/lipase ESM1 16 54 188 7.6/44.3 Defense Secreted Defence responses to bacterium glucosinolate catabolic process response to cold
53 Q9SZJ5 Serine hydroxymethy-transferase 18 46 187 8.1/57.5 Metabolism/Defense Mitochondrion Gly and Ser metabolism Hypersensitive Response tetrahydrofolate interconversion response to abiotic stress
54 Q9LJG3 GDSL esterase/lipase ESM1 6 28 95 7.6/44.3 Defense Secreted Responses to bacterium glucosinolate catabolism response to cold

* Mascot search results: line 1: Matched Peptides; lane 2: Sequence Coverage (%); Line 3: Score; Line 4: Mixed score.