Figure 4.

Caffeine inhibits the formation of chromosome bridges. HCT116/v-Src cells were cultured with Dox for three days at the indicated concentrations. The cells were treated with or without 2 mM caffeine for one day before fixation or lysate preparation. (A) The percentage of anaphase and telophase cells with a chromosome bridge were plotted. The results represent the mean ± S.D. from three independent experiments. p values were calculated using a two-tailed Student’s t-test; (B) Whole cell lysates were subjected to a Western blot analysis. Blots were probed with anti-phosphotyrosine (pTyr), anti-Src (pY416), and anti-G3PDH (loading control) antibodies; (C) Whole cell lysates were subjected to a Western blot analysis. Blots were probed with anti-Chk1 pSer345 and anti-G3PDH (loading control) antibodies. In B and C, a representative result of two independent experiments is shown.