Figure 3.

Cypermethrin leaded to G1 cell cycle arrest in RAW 264.7 cells. RAW 264.7 cells were treated with cypermethrin for 48 h (A). After treatment, cells were harvested and processed for cell cycle distribution analysis using flow cytometry; (B) Expression levels of p53 and G1 phase cell cycle regulators in RAW cells treated with cypermethrin. RAW cells were treated with or without cypermethrin for 48 h. After treatment, cells were harvested and cell lysates were subjected to the analysis of cell cycle regulatory proteins of G1-phase and p53 using western blot analysis; and (C) Cells were pretreated with 5 mM NAC for 1 h and treated with cypermethrin for 48 h. Cells were analyzed for cell cycle distribution. Quantitative data shown for G1, G2 and S phase cell population were representive of three independent experiments. * p < 0.05 as compared with vehicle alone.