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. 2016 Jun 14;17(6):903. doi: 10.3390/ijms17060903

Figure 4.

Figure 4

Flow cytometry analysis of CD133, CXCR4 and Ki-67 expression in chemonaive HT-29 cells, HT-29/5-FU cells on the third day after 5-FU removal from the culture, and in HT-29/5-FU cells passaged and cultured for four days with or without vitamin D analogues. CD133 and CXCR4 expression is shown for total chemonaive HT-29 cells and total HT-29/5-FU cell population. Ki-67 expression was analyzed in total cell populations and in gates set for CD133+ cells or CXCR4+ cells. Data from one out of three experiments with similar results are shown. Histograms showing analysis of samples labeled with fluorochrome-conjugated unspecific antibodies of respective isotypes, used as a negative control, are shown in the top row. Abbreviations: IgG—immunoglobulin G, AF 647—Alexa Fluor 647.