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. 2016 Jun 8;17(6):905. doi: 10.3390/ijms17060905

Figure 5.

Figure 5

Effects of SB203580 on TBI-induced increase in ROS in HPCs and HSCs. Mice were treated with vehicle or SB203580 70 days after exposure to 6 Gy TBI, as described in the experimental section. BMNCs were collected from the mice 80 days after 6 Gy TBI, and HSCs were isolated from BMNCs by cell sorting. (A,B) Analysis of the expression of p-p38 and p16 in HSCs. Left: representative photomicrographs of p-p38/p16 and DAPI nuclear staining immunostaining in isolated HSCs are shown; Right: the percentages of p-p38/p16 positive HSCs are presented as the means ± SD (n = 3). Magnification: 10×; (C) The levels of p16 mRNA expression in the HSCs were analyzed via RT-PCR. The data are presented as the means ± SEM of the fold changes compared with the control (n = 3). a p < 0.05 vs. control., b p < 0.05 vs. TBI + vehicle.