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. 2016 Jun 9;17(6):910. doi: 10.3390/ijms17060910

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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CLC-2 knockdown inhibits transforming growth factor (TGF)-β1-induced migration in cultured Human conjunctival fibroblasts (HConF). (A) Confluent HconF were wounded with a sterile 200-µL pipette tipand each scratch was imaged at 0 and 24 h after wounding, migratory distance were recorded to determine the migratory ability of HconF (B) transwell filters were stained with crystal violet to visualize migrated cells (20× magnification), the number of HconF cells in the lower layer were recorded to determine the migratory ability of HconF. Data are presented as the mean ± SD (n = 4); siRNA-NC: mutant CLC-2 siRNA; ** p < 0.01 vs. control; ## p < 0.01 vs. TGF-β1 in ANOVA.