Figure 4.

Effects of MLA on the CFU-GM frequency and the CFU-S number. (A) The CFU-GM frequency of BMMNCs. Before the mice were exposed to 6 Gy TBI, they were treated with either vehicle or MLA (10 mg/kg) each day three times. Sham-irradiated control mice and MLA-treated mice were also included. BMMNCs were collected and counted after the mice were euthanized nine days after exposure to 6 Gy TBI. The cells were cultured in MethoCult GF M3534 methylcellulose medium for CFU-GM analysis; (B) Number of CFU-S. The number of CFU-S per spleen was counted after the spleens were fixed in Bouin’s solution for 24 h. Results are expressed as the mean ± SEM (n = 5 for each group). The p value shows the difference compared with the IR group.