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. 2016 Jun 14;17(6):935. doi: 10.3390/ijms17060935

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of MLA on the levels of ROS. (A) A representative flow cytometry graph: The BMMNCs(P1) were firstly gated by Strepevidin–Percp and C-kit-APC, then the HPC (lin⁻ c-kit⁺ Sca-1⁻, R2) and HSC (lin⁻ c-kit⁺ Sca-1⁺, R3) cells were gated in the lineage negative cells (Strepevidin⁻, R1), and the ROS levels in these cells were further analyzed; (B) ROS levels in BMMNCs; (C) ROS levels in HPCs; (D) ROS levels in HSCs. Before the mice were exposed to 6 Gy TBI, they were treated with either vehicle or MLA (10 mg/kg) each day three times. Sham-irradiated control mice and MLA-treated mice were also included. BMMNCs were collected and detected according to the Methods. The results are expressed as the mean ± SEM. The p value shows the difference compared with the IR group.

Effects of MLA on the levels of ROS. (A) A representative flow cytometry graph: The BMMNCs(P1) were firstly gated by Strepevidin–Percp and C-kit-APC, then the HPC (lin⁻ c-kit⁺ Sca-1⁻, R2) and HSC (lin⁻ c-kit⁺ Sca-1⁺, R3) cells were gated in the lineage negative cells (Strepevidin⁻, R1), and the ROS levels in these cells were further analyzed; (B) ROS levels in BMMNCs; (C) ROS levels in HPCs; (D) ROS levels in HSCs. Before the mice were exposed to 6 Gy TBI, they were treated with either vehicle or MLA (10 mg/kg) each day three times. Sham-irradiated control mice and MLA-treated mice were also included. BMMNCs were collected and detected according to the Methods. The results are expressed as the mean ± SEM. The p value shows the difference compared with the IR group.