Effects of MLA on the expression of NOX4. (A) A representative flow cytometry graph: The BMMNCs(P1) were firstly gated by Strepevidin–Percp and C-kit-APC (R1), then the HPC (lin− c-kit+ Sca-1−, R2) and HSC (lin− c-kit+ Sca-1+, R3) cells were gated in the lineage negative cells (Strepevidin−, R1), and the NOX4 expression in these cells were further analyzed; (B) NOX4 expression in BMMNCs; (C) NOX4 expression in HPCs; (D) NOX4 expression in HSCs. Before the mice were exposed to 6 Gy TBI, they were treated with either vehicle or MLA (10 mg/kg) each day three times. Sham-irradiated control mice and MLA-treated mice were also included. BMMNCs were collected and detected according to the Methods. Results are expressed as the mean ± SEM. The p value shows the difference compared with the IR group.