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. 2016 Jun 16;17(6):953. doi: 10.3390/ijms17060953

Figure 1.

Figure 1

Experimental plan of in vitro cell exposure. (a) Non-tumorigenic murine alveolar type II epithelial cells (C10) were exposed to 100% O2 for 8 h only (O2), 0.25 Gy ionizing γ-radiation (IR) only, or a double-hit combination of both challenges (O2 + IR) followed by 16 h of normoxia (21% partial pressure of oxygen (pO2)) (1 cycle). This was repeated for 2 cycles and cells were harvested at the end of each 24 h cycle: 1 and 2 cycles of exposure corresponding to 24 and 48 h, respectively; (b) Images displaying the experimental setup of in vitro cell exposure. Pictured are the chambers holding the 6 cm cell culture Petri dishes and the gas manifold allowing for O2 exposure.