Table 4.
Primer sequences of mRNA used for qRT-PCR analysis.
| Gene | Forward (5’ to 3’) | Reverse (5’ to 3’) |
|---|---|---|
| Ppm1f | GCTTCTTCAACTGCCTTTGG | CCATGACCATCAAACACAGC |
| 5830404H04Rik | GCTGGCTCATTTTCTTCAGG | CGGAAGAAAAGCACCATCAT |
| Slc1a2 | TCTGAGGAGGCCAATACCAC | TTCATCCCGTCCTTGAACTC |
| Prom2 | GTGTGACATGATGGCTGACC | ACCCTGGGGATATGGAAAAG |
| Pkaa2 | CGGCGCCTTTCCTTGAATAT | GGCCTGTTCCTCACGGTATTA |
| Marcksl1 | GGCAGCCAGAGCTCTAAGG | TCACGTGGCCATTCTCCT |
| Khk | AGCCTCATGGAAGAGAAGCA | GGAGGTCATCCAGGACAAAA |
| Gabra3 | ATGTGGCACTTTTATGTGACCA | CCCCAGGTTCTTGTCGTCTTG |
| Col9a3 | CAAGGATGGCATTGATGGAG | CAGACCATCTACACCAGGCAGT |
| Fbxl22 | ATGGGCAAGCAGGTTAAATG | CACGCGAACAGAACAGAAAA |
| Bmp4 | GAGGAGGAGGAAGAGCAGAG | TGGGATGTTCTCCAGATGTT |
| Csnk1g1 | CAGTGGTTTTCACCTTCTGG | AGGCCTTCACCTGCACTG |
| Csnk1g2 | TACTACTTCGGCCCTTGTGG | GGCTTCACGTCACGGTAGAT |
| Gas1 | TAAATTGCTTGTGACCACTG | GAGCATTACCGATGGATAGA |
| Gli1 | TGTGTGAGCAAGAAGGTTGC | ATGGCTTCTCATTGGAGTGG |
| Gli3 | GCAACCTCACTCTGCAACAA | CCTTGTGCCTCCATTTTGAT |
| Hhip | GGTCACATCTTGGGATTTGG | TCCATTGTGAGTCTGGGTCA |
| Ihh | CAATCCCGACATCATCTTCA | AGTTCAGACGGTCCTTGCAG |
| Prkacb | TCTTTCCTGCGTCATCAGTG | AAGGGAGCACTGGTCAGAGA |
| Stk36 | CCAGGAGAGTAGCAGCATCC | TGTCTGCTCGGAATCATCTG |
| Sufu | TCCAGGTTACCGCTATCGTC | TCCACTGTTGGGCTGAATGT |
| Wnt6 | TGTCAGTTCCAGTTCCGTTTCC | GCTGCGGTGATTGCAAACA |
| Wnt3a | GGCTCCTCTCGGATACCTCT | GGGCATGATCTCCACGTAGT |
| Wnt3 | CAAGCACAACAATGAAGCAGG | TCGGGACTCACGGTGTTTC |
| Wnt10b | ACGACATGGACTTCGGAGAGAAGT | CATTCTCGCCTGGATGTCCC |
| Wnt9a | GGCACAGGGTTACAAACAAC | GGACAGAGGCAACTGAGAAA |
| Wnt11 | ATGTGCGGACAACCTCAGCTA | CGCATCAGTTTATTGGCTTGG |