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. 2016 Jun 17;17(6):963. doi: 10.3390/ijms17060963

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of hASCs. (A) hASCs were incubated with adipogenic, chondrogenic, and osteogenic differentiation medium for 21 days. The efficiencies of differentiation were determined by histological staining as Oil Red O staining, Alcian Blue staining, and von Kossa staining, respectively (scale bar = 50 μm); (B) the mRNA expression of differentiation marker was measured by qPCR after 21 days of induction. The data are shown as the mean ± SEM (n = 3, **: p < 0. 005, ***: p < 0.0005); (C) the migration of Ud-ASC, Ad-ASC, Cd-ASC, and Od-ASC were investigated using Radius™ 24-Well Cell Migration Assay. The broken line circles indicate the rim of the cells prior to migration; (D) the quantitative data of the migration area was calculated by ImageJ software (n = 3); and (E) the proliferation of Ud-ASC, Ad-ASC, Cd-ASC, and Od-ASC was measured using CCK-8 kit. The data are shown as the mean ± SEM (n = 3, Ud-ASC vs. Cd-ASC, *: p < 0. 05).

Characterization of hASCs. (A) hASCs were incubated with adipogenic, chondrogenic, and osteogenic differentiation medium for 21 days. The efficiencies of differentiation were determined by histological staining as Oil Red O staining, Alcian Blue staining, and von Kossa staining, respectively (scale bar = 50 μm); (B) the mRNA expression of differentiation marker was measured by qPCR after 21 days of induction. The data are shown as the mean ± SEM (n = 3, **: p < 0. 005, ***: p < 0.0005); (C) the migration of Ud-ASC, Ad-ASC, Cd-ASC, and Od-ASC were investigated using Radius™ 24-Well Cell Migration Assay. The broken line circles indicate the rim of the cells prior to migration; (D) the quantitative data of the migration area was calculated by ImageJ software (n = 3); and (E) the proliferation of Ud-ASC, Ad-ASC, Cd-ASC, and Od-ASC was measured using CCK-8 kit. The data are shown as the mean ± SEM (n = 3, Ud-ASC vs. Cd-ASC, *: p < 0. 05).