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. 2016 Jun 17;17(6):963. doi: 10.3390/ijms17060963

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Priming effect of Col II for chondrogenic differentiation of hASCs. (A) The proliferation of Cd-ASC was determined on normal and Col II-coated plates by CCK-8 assay. The data are shown as the mean ± SEM (n = 3, ns: p = 0. 3922); (B) the chondrogenic differentiation was confirmed by Alcian Blue staining after 0, 7, and 21 days of induction (scale bar = 500 μm); and (C) the GAG contents were assessed by Alcian Blue extraction with 8 M of guanidine hydrogen chloride. The absorbance was measured at 610 nm by microplate reader. The data are shown as the mean ± SEM (n = 3, *: p < 0.05).

Priming effect of Col II for chondrogenic differentiation of hASCs. (A) The proliferation of Cd-ASC was determined on normal and Col II-coated plates by CCK-8 assay. The data are shown as the mean ± SEM (n = 3, ns: p = 0. 3922); (B) the chondrogenic differentiation was confirmed by Alcian Blue staining after 0, 7, and 21 days of induction (scale bar = 500 μm); and (C) the GAG contents were assessed by Alcian Blue extraction with 8 M of guanidine hydrogen chloride. The absorbance was measured at 610 nm by microplate reader. The data are shown as the mean ± SEM (n = 3, *: p < 0.05).